基于液相质谱的单克隆抗体全长 De Novo 测序分析测试服务是借助全新一代抗体鉴定平台提供的一项专业分析服务。该服务的核心技术源于基于质谱数据的从头测序算法以及蛋白质谱数据分析软件,整合了目前世界上最先进的液相质谱技术以及质谱数据分析技术。

我们提供整套抗体全长蛋白测序分析测试服务,从实验到数据解析到最终报告,您只需提供不少于200 µg的纯蛋白 (纯度要求≥90%),即可获取关于此样品的全长蛋白序列准确信息。

样品要求:纯度>90%,样品量>200 µg;IgG单抗,其他类型请提前告知;种(人源、鼠源为常规物种,其他物种请提前告知,样品中其他杂质可能成分及比例,参考序列 (如有)

服务周期:4-6 周

单克隆抗体全长 De Novo 测序分析测试服务工作流程

分析测试服务报告 (涵盖,但不限于以下内容)

1. 抗体样品描述

2. 各种缩写说明

3. 序列覆盖度

4. 详细结果报告:

重链:1)准确分子量(去糖/不去糖)

          2)氨基酸序列(100%序列覆盖)

          3)肽段-谱图匹配结果(质控条件1%FDR)

          4)可变区肽段列表(序列、RT、m/z、二级谱图等)

          5)亮氨酸/异亮氨酸差异

轻链:1)准确分子量(去糖/不去糖)

          2)氨基酸序列(100%序列覆盖)

          3)肽段-谱图匹配结果(质控条件1%FDR)

          4)可变区肽段列表(序列、RT、m/z、二级谱图等)

          5)亮氨酸/异亮氨酸差异

其余内容可增值定制,如N-糖谱、轻重链可变区PTM修饰分析等


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