Rewiring of the Proteome and Phosphoproteome in Mammalian Cells Under Selection and Stress
Yansheng Liu (Yale University School of Medicine)
To understand the molecular basis of proteotype rewiring and evolution in mammalian cells, we normally measure both the relevant proteomes and phosphoproteomes in the Liu Lab. As examples, in this presentation, we will mainly discuss how we utilize DIA-MS and cutting-edge software solutions such as Spectronaut in analyzing two intriguing biological questions. The first is how we reveal the quantitative principles governing proteotype diversity and co-evolution in mammalian cells across eleven species. The second is how phosphoproteomics-DIA could uncover the heterogeneity of colorectal cancer cell responses to a widely prescribed antidiabetic drug.