悟空云平台交流第27期---数据前处理各要点
近期发现很多老师和同学在我们交流群里（QQ: 688728477）贴出使用悟空云时报错，经过交流发现，绝大多数报错的原因是因为其没有对数据做好前处理工作，因此本期我们再次跟大家强调一下数据前处理需要注意的一些关键点，希望对大家有所帮助，也希望大家仔细观看，认真做好笔记。
本系列视频会收录在我们公众号菜单：技术动态->悟空云系列讲座
使用悟空云平台，可在文章中如下描述：
1) 比如在正文Methods部分：We use 'Wu Kong' platform (https://www.omicsolution.com/wkomics/main/) for relative xxx analysis.
2) 比如在致谢(Acknowledgements)部分：Thank Dr. Shisheng Wang (West China Hospital, Sichuan University) and Dr. Chengpin Shen (Omicsolution Co., Ltd) for giving some advices about data analysis and 'Wu Kong' platform (https://www.omicsolution.com/wkomics/main/) for relative xxx analysis.
目前基于悟空平台开发的分析工具已发表以下文章：
1. (2020) Shisheng Wang , Wenxue Li, Liqiang Hu, Jingqiu Cheng, Hao Yang, Yansheng Liu, NAguideR: performing and prioritizing missing value imputations for consistent bottom-up proteomic analyses, Nucleic Acids Research, gkaa498. (2021 IF: 19.16).
2. (2022) Huilin Yu, Haiyu Luo, Luping Chang, Shisheng Wang （共一作） , ..., Hao Yang, Wenying Fan & Bing-Qiao Zhao, The NEDD8-activating enzyme inhibitor MLN4924 reduces ischemic brain injury in mice. Proceedings of the National Academy of Sciences, 119(6). (2021 IF: 12.779).
3. (2021) Shisheng Wang , et al. StatsPro: Systematic integration and evaluation of statistical approaches for detecting differential expression in label-free quantitative proteomics, Journal of Proteomics, 10.1016/j.jprot.2021.104386. (2020 IF: 4.04).
4. (2021) Shisheng Wang , Yi Zhong, Jingqiu Cheng, Hao Yang, EnrichVisBox: A Versatile and Powerful Web Toolbox for Visualizing Complex Functional Enrichment Results of Omics Data, Journal of computational biology, 10.1089/cmb.2020.0564. (2020 IF: 1.479).
5. (2020) Shisheng Wang , Hongwen Zhu, Hu Zhou, Jingqiu Cheng and Hao Yang. MSpectraAI: a powerful platform for deciphering proteome profiling of multi-tumor mass spectrometry data by using deep neural networks. BMC Bioinformatics 21, 439 (2020). (2019 IF: 3.242).
6. (2019) Shisheng Wang , Yue Cai, Jingqiu Cheng, Wenxue Li, Yansheng Liu and Hao Yang. motifeR: An Integrated Web Software for Identification and Visualization of Protein Post‐Translational Modification Motifs. Proteomics: 201900245. (2018 IF: 3.106).
7. (2019) Shisheng Wang and Hao Yang. pseudoQC: A Regression‐Based Simulation Software for Correction and Normalization of Complex Metabolomics and Proteomics Datasets. Proteomics: 1900264. (2018 IF: 3.106).
8. (2018) Shisheng Wang , ..., and Meng Gong. MetaboGroupS: A Group Entropy-Based Web Platform for Evaluating Normalization Methods in Blood Metabolomics Data from Maintenance Hemodialysis Patients. Anal Chem 90, 11124-11130. (2017 IF: 6.042).
9. (2018) Shisheng Wang , ..., and Hao Yang. MixProTool: A Powerful and Comprehensive Web Tool for Analyzing and Visualizing Multigroup Proteomics Data. Journal of computational biology : a journal of computational molecular cell biology 25, 1123-1127. (2017 IF: 1.191).
10. (2018) Qing-Run Li, Shi-Sheng Wang(共一作) , Hong-Wen Zhu, Fang-Ying Xia, Peter Roepstorff, Jia-Rui Wu, and Rong Zeng. Data-Independent Acquisition Mass Spectrometry to Localize Phosphosites. bioRxiv: 464545.
同时悟空平台整合上百个分析模块，包括且远不止以下功能
Circulating proteomic panels for risk stratification of intracranial aneurysm and its rupture
Yueting Xiong, ...,Hebin Liu,...,Xiaohui Liu EMBO Mol Med, IF12.3,2022
High-throughput site-specific N-glycoproteomics reveals glyco-signatures for liver disease diagnosis
Zhenyu Sun, et al. National Science Review , IF19,2022 Multi-Omic Profiling of Multi-Biosamples Reveals the Role of Amino Acid and Nucleotide Metabolism in Endometrial Cancer
Runqiu Yi, Liying Xie, Xiaoqing Wang, Chengpin Shen, Xiaojun Chen*, Liang Qiao*，Frontiers in Oncology，IF6.3,2022
Mesoporous Silica as Sorbents and Enzymatic Nanoreactors for Microbial Membrane Proteomics
J. Zhao, R. Jian, Y. Wang, B. Yang, D. Zhao, C. Shen, L. Qiao*, B. Liu*, ACS Applied Materials & Interfaces, IF9,2021
Direct MALDI-TOF Profiling of Gingival Crevicular Fluid Sediments for Periodontitis Diagnosis,
J. Yi, Y. Shen, Y. Yang, C. Shen, B. Liu, L. Qiao*, Y. Wang*, Talanta, IF5,2021
Proteomic and Metabolic Elucidation of Solar-Powered Biomanufacturing by Bio-Abiotic Hybrid System.
Rutan Zhang, ..., Chengpin Shen, ..., Liang Qiao Chem IF18,2020
In silico spectral libraries by deep learning facilitate data-independent acquisition proteomics.
Yi Yang, ...,Chengpin Shen,... and Liang Qiao Nature Communications IF13,2020
Metaproteomics characterizes human gut microbiome function in colorectal cancer.
Shuping Long, ..., Chengpin Shen,.. and Liang Qiao npj Biofilms and Microbiomes IF5,2020