--- title: "Spectronaut 15更新介绍" author: "omicsolution" account: "omicsolution" published_at: "2021-06-16T15:52:26+08:00" captured_at: "2026-08-24T11:50:16.224903+08:00" source_url: "https://mp.weixin.qq.com/s?__biz=MzI2MTI5NDAzNg==&mid=100002267&idx=1&sn=a1e82d31c6c025cc36bdb980adf2a4e2&chksm=6a5ddb605d2a52760b95f28a94d440d2ad539c94c3a9773fcf8ada0d23b845005d9c1d5dfcb6#rd" --- # Spectronaut 15更新介绍 ![图片 1](images/001.png) Spectronaut 15 带着众多性能改进、功能更新于6月15日晚正式发布。在新一代深度学习算法的加持下，DIA数据分析的性能和DDA的差距进一步扩大。 新版本详细介绍视频（中文校正版）： Mann课题组单细胞DIAPASEF介绍（中文自动翻译）及Q&A： Spectronaut 15 主要更新内容如下 - 定性能力提升： 谱图库DIA和directDIA结果均有明显提升 - 定量改进：整合MaxLFQ算法，提升定量准确性 - 全面的PTM分析功能：PTM位点分析全面升级，你想要的都有了 - 改善用户体验：增加大量DIA数据可视化功能 - 深度学习：改进机器学习算法显著提升免疫多肽和离子淌度数据分析能力 定性能力提升 相对于Spectronaut14来说，新版本在显著提升了DIA-PASEF数据的定性能力，基于谱图库或directDIA均有15-30%的提升。非DIA-PASEF数据实现了5%-20%的提升。 提升灵敏度的同时，SN15引入基于单个run的FDR控制算法，能够大显著改进FDR的准确性同时不牺牲灵敏度。 ![图片 2](images/002.png) 定量改进 DIA分析的核心痛点是定量的准确性。Spectronaut15中加入了MaxLFQ算法，在CV不变的情况下，进一步提升了可定量的母离子比例。 ![图片 3](images/003.png) 全面的PTM分析功能 PTM定量分析，位点置信度及位点定量分析是目前蛋白质组学中的核心关切点，而大部分分析软件都依赖于研究者自行进行位点拆分、定量和解读。 SN15的PTM专属分析流程为您带来一键式的位点置信度、定量、差异分析功能，同时全部支持可视化查看，为您的修饰定量插上翅膀。 相关详细介绍可以参考官方简介视频： DIA数据可视化改进 对于商业化数据分析软件来说，可视化界面的使用体验是其相对于免费软件的重要优势之一。 在SN15中，我们加入了全新的以蛋白为核心的结果查看、差异展示和离子淌度可视化界面。 详细用户界面改进参见官方视频介绍： Spectronaut用户请连接上互联网后点击About界面自行更新。 如需进一步帮助欢迎和我们联系。 详细更新日志： More Identifications - Improved identification with new scores, especially for short gradients - Up to 35% more precursor identifications for directDIA with diaPASEF - Up to 10% more precursor identifications for directDIA in general - Up to 30% more precursor identifications for short gradient DIA analysis with library - Up to 10% more precursor identifications for DIA analysis with library in general Accurate Quantification - Improved default settings for quantification - New MaxLFQ based protein quantification - Up to 15% more protein identifications with CVs below 10% in controlled quantitative experiments Improved Ion Mobility Support - Ion Mobilogram visualization for dia-PASEF - Ion Mobilogram based manual peak refinement - Up to 30% faster processing of dia-PASEF data - Added optimal support for high-sensitive py5 dia-PASEF method General DIA Analysis - New Run-wise protein FDR calculation and filtering - New peptide posterior error probability (PEP) filtering - Improved flexibility for directDIA search settings - Improved multi-channel quantity summarization - Command line support for SNECombine workflow - New “Method Evaluation” workflow with directDIA Comprehensive PTM Analysis - Site collapse calculation - Site regulation analysis - Modification specific normalization filter for enrichment workflows - New PTM site report Pulsar Database Search Engine - Improved performance for large protein databases (> 2 GB) with Pulsar - Improved identification and performance for unspecific searches Deep-learning Augmented Improvements - Prediction for ion mobility (1/K0) during library generation - Improved fragment prediction for unspecific peptides New Post Analysis Feature s - Added PCA analysis - Added custom selection for protein rank plot - Added custom selection for volcano plots - New PTM Analysis node Improved User Experience and Visualization - Improved Protein Coverage plot - PTM support - Peptide tooltip and highlighting - New sub-perspective “Protein Grid View” in the analysis perspective - Improved XIC Alignment plots with support for unlimited number of runs - Detachable perspective and plots for side-by-side visualization - Improved XIC grid overview plots - One-Click tree navigation in plots - Improved plot and tree filter selection - Quick-Action bar for analysis perspective - Improved warning messages with actionable links - Improved UI for analysis log - Updated Analysis Summary plot for easier access Other Changes - Report Perspective Changes - New PTM site level report when performing PTM localizationPage - DIA Analysis Settings Changes - Default row filtering for quantification changed from Q-value to Q-value Sparse - Default quantification strategy changed from Quant 2.0 to Auto, where it will use MaxLFQ if analyzing less than 500 runs - Default normalization strategy changed from Global Normalization to Auto, where it will use Local Normalization if analyzing less than 500 runs - New PTM workflow node in the setting - directDIA Analysis Settings Changes: - New Method Evaluation setting in directDIA settings → Workflow → Method Evaluation - Distinguish between Pulsar and DIA analysis settings for directDIA for more flexibility www.omicsolution.com 若有任何疑问，欢迎邮件或来电咨询： marketing@omicsolution.com support@omicsolution.com +86-18221381405